anti smad7  (Proteintech)

Journal: The Journal of Headache and Pain

Article Title: Electroacupuncture alleviates central post-stroke pain in rats by modulating miR-21-5p/TGF-β/Smads pathway

doi: 10.1186/s10194-025-02247-1

Figure Lengend Snippet: miR-21-5p targets and suppresses the expression of Smad7. A . Diagram of the binding sites between miR-21-5p and the 3’ UTR of Smad7. B . Double luciferase reporter assays demonstrate that miR-21-5p targets the 3’ UTR of Smad7. C . WB expression of Smad7 in the VPL (ventral posterolateral) in different groups ( n = 4). D . WB expression of Smad7 in CTX-TNA rat astrocyte cell line with inhibited miR-21-5p ( n = 4). E . WB expression of Smad7 in CTX-TNA rat astrocyte cell line overexpressing miR-21-5p ( n = 4)

Article Snippet: Rat brain tissue sections, previously embedded in paraffin, underwent incubation with GFAP (1:250, Abcam, UK), IBA1 (1:250, CST, USA), and Smad7 (1:250, Proteintech, China) antibodies.

Techniques: Expressing, Binding Assay, Luciferase

Journal: The Journal of Headache and Pain

Article Title: Electroacupuncture alleviates central post-stroke pain in rats by modulating miR-21-5p/TGF-β/Smads pathway

doi: 10.1186/s10194-025-02247-1

Figure Lengend Snippet: Smad7 is a critical target in the CPSP. A . Schematic diagram of the viral vector construction and injection of Smad7 knock down and overexpression (KD represents Smad7 knock down; OE represents Smad7 overexpression). B - D . The effectiveness of Smad7 KD was confirmed by immunofluorescence (b and c, n = 3) and RT-PCR (d, n = 4). E - G . The effectiveness of Smad7 OE was confirmed by immunofluorescence ( E and F , n = 3) and RT-PCR (g, n = 4). H - I . Pain threshold evaluation ( n = 6 or 8 or 11). J . Immunofluorescence labeling for astrocyte marker GFAP (green), microglial marker IBA1 (green), DAPI (blue) for cell nuclei, all scale bars represent 100 μm as indicated. K - L . WB analysis of GFAP and IBA1 ( n = 3). M . Cytokine expression was determined by ELISA ( n = 8). **** P < 0.0001 versus Sham; & P < 0.05 versus CPSP; #### P < 0.0001 versus CPSP. ns, P > 0.05 not significant. Statistical analysis was performed using one-way or two-way ANOVA as appropriate

Article Snippet: Rat brain tissue sections, previously embedded in paraffin, underwent incubation with GFAP (1:250, Abcam, UK), IBA1 (1:250, CST, USA), and Smad7 (1:250, Proteintech, China) antibodies.

Techniques: Plasmid Preparation, Injection, Knockdown, Over Expression, Immunofluorescence, Reverse Transcription Polymerase Chain Reaction, Labeling, Marker, Expressing, Enzyme-linked Immunosorbent Assay

Journal: The Journal of Headache and Pain

Article Title: Electroacupuncture alleviates central post-stroke pain in rats by modulating miR-21-5p/TGF-β/Smads pathway

doi: 10.1186/s10194-025-02247-1

Figure Lengend Snippet: Smad7 is the target through which electroacupuncture exerts its analgesic effects. A . Pain threshold evaluation ( n = 6 or 10). B . Immunofluorescence labeling for Smad7 (green), astrocyte marker GFAP (green), microglial marker IBA1 (green), DAPI (blue) represents cell nuclei, scale bars represent 100 μm as indicated. C - D . WB analysis of GFAP, IBA1 and Smad7 ( n = 3). E . Cytokine expression was determined by ELISA ( n = 6 or 8). *** P < 0.001 versus Sham; **** P < 0.0001 versus Sham; & P < 0.05 versus CPSP + EA; && P < 0.01 versus CPSP + EA; &&&& P < 0.0001 versus CPSP + EA. Statistical analysis was performed using one-way or two-way ANOVA as appropriate

Article Snippet: Rat brain tissue sections, previously embedded in paraffin, underwent incubation with GFAP (1:250, Abcam, UK), IBA1 (1:250, CST, USA), and Smad7 (1:250, Proteintech, China) antibodies.

Techniques: Immunofluorescence, Labeling, Marker, Expressing, Enzyme-linked Immunosorbent Assay

Journal: The Journal of Headache and Pain

Article Title: Electroacupuncture alleviates central post-stroke pain in rats by modulating miR-21-5p/TGF-β/Smads pathway

doi: 10.1186/s10194-025-02247-1

Figure Lengend Snippet: Electroacupuncture exerts analgesic effect by influencing miR-21-5p/Smad7, thereby further regulating the TGF-β/Smads pathway in rats with CPSP. A - B . WB expression of TGF-β1, p-Smad2 and p-Smad3 in the VPL (ventral posterolateral) region of rat brain tissue in different groups ( n = 3). C . Diagram of the mechanism of electroacupuncture exerts analgesic effect on CPSP

Article Snippet: Rat brain tissue sections, previously embedded in paraffin, underwent incubation with GFAP (1:250, Abcam, UK), IBA1 (1:250, CST, USA), and Smad7 (1:250, Proteintech, China) antibodies.

Techniques: Expressing